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Session: Cancer
Session starts: Friday 25 January, 10:30
Presentation starts: 10:30
Room: Lecture room 559

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Laura van Huizen (Vrije Universiteit Amsterdam)
Nikolay Kuzmin (Vrije Universiteit Amsterdam)
Ellis Barbé (VU Medisch Centrum Amsterdam)
Susanne van der Velde (VU Medisch Centrum Amsterdam)
Elisabeth te Velde (VU Medisch Centrum Amsterdam)
Marie Louise Groot (Vrije Universiteit Amsterdam)

Abstract:
Background For a patient with breast cancer, fast diagnosis and precise excision of breast tumor tissue is important. The gold standard to assess excised breast tissue is histopathology, which takes at least 16 hours. Alternative techniques are therefore required that can assess breast tissue with a speed that enables ‘live’ feedback to the surgeon while she/he operates. HHG Microscopy A technique that meets these requirements is higher harmonic generation microscopy (HHGM), a novel imaging technique, which is non-invasive, label-free and provides 3D images with a high, sub-cellular resolution, within seconds [1]. HHGM reveals tissue contrast provided by interfaces and noncentrosymmetric molecular structures via generation of second and third optical harmonics (SHG/THG). Results 3D images of freshly excised and unprepared human healthy and tumor breast tissue showed that the combination of THG and SHG microscopy revealed the key breast components – lobules, ducts, fat tissue, connective tissue, blood vessels, and peripheral nerves. All THG/SHG images were in good agreement with H&E histology. Furthermore, the experiments showed that HHGM is able to reveal most of the pathological breast features. Thus, HHGM has a high added value for fast label-free assessment of fresh breast tumor tissue. Possible applications are intra-operative assessment and in-situ analysis, which makes HHGM a promising technique. [1] N.V. Kuzmin, et al. Biomed. Opt. Exp., 7, 1889–1904 (2016)